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KMID : 0378019770200070077
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New Medical Journal
1977 Volume.20 No. 7 p.77 ~ p.82
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Changes of the Lactate Dehydrogenase (LDH) Isoenzyme Patterns in the Retina and Cerebrum of the Rabbit Embryos
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Abstract
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Glucose oxidation in the adult retina is more than in other animal tissues, including brain slices (Di Pietro and Weinhouse, 1959; Adler, 1965). Aerobic and anaerobic lactic acid. production is also extremely, rapid(Brotherton, 1962). Thus, the control of the fate of pyruvate in this tissue would seem to be of special importance.
Developmental studies in retina have shown that after birth, there is a substantial increase in anaerobic glycolysis and a much higher glucose oxidation via the Krebs cycle (Graymore, 1960; Noell, 1965; Cohen and Noell, 1960).
Pasantes-Morales (1972) found that the proportion of M-type lactate dehydrogenase subunits decreases significantly in brain and retina with development and a marked increase in the H-type subunits is observed in retina.
In the present investigation, we have been investigated the LDH isoenzyme patterns in the retina and cerebrum of the rabbit embryos by cellulose acetate electrophoresis (Kohn, 1958). A similar investigation was carried out in cardiac and skeletal muscles of the rabbit embryos. Tissue samples were collected from the 22, 24, 26, 27, 29, 30-days embryos and the neonatal rabbit after birth.
The electrophoretic patterns of LDH isoenzymes in retina and cerebrum is illustrated in Figs.1 and 2. We have observed only four bands in the rabbit retina and -cerebrum from 22-day-old embryos. In the zymograms of the rabbit retina and cerebrum, the highest activities correspond to LDH3 and LDH4 bands and in the retina LDH2 band was detected as a faintly visible and measurable coloured band. On the other hand, LDH1 band was recessive in the rabbit embryos. These characteristic patterns were maintained throughout the intrauterine period. During embryonal stages the enzyme patterns of the cerebrum were similar to those of the retina.
In the cardiac muscle four defined bands -of enzyme activities were observed, which were corresponding to the four LDH isoenzymes. The bands are numbered 2-5 in order of increasing mobility towards the cathods. The most :remarkable activities were :confirmed as LDH3 and LDH2 bands. In the skeletal muscle, the LDH5, LDH4 and LDH3 bands of M-type LDH isoenzyme is observed in all experimental stages.
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